fusion expression vector meaning in English
融合表达载体
Examples
- So , we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein
为此将emt l氨基末端基因克隆入融合表达载体prsetb中，转化大肠杆菌bl 21 ，经iptg诱导，表达his6 emt l融合蛋白，表达产物分子量约为24ku 。 - Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ) , then subcloned into the eucaryote expression vector pcdna3 . 1 ( + ) after sequence analysis
将halr基因克隆到原核融合表达载体pet28a ( + )上，序列分析后亚克隆到真核表达载体pcdan3 . 1 ( + )上。 - Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene , and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector . the positive pbv220 - endostatin was transformed into dh5a , and expressed mouse endostatin protein successfully . this study is the basis of the endostatin gene - engineering production
本文成功克隆鼠内皮抑素基因，构建了内皮抑素基因的原核表达载体pbv220一endostatin ，该载体为非融合性表达载体，将pbv22o一endostatin成功转化到大肠杆菌表达菌dh5a中，并诱导表达了鼠内皮抑素蛋白，为基因工程方法生产内皮抑素奠定一定基础。 - The total rna was extracted from human normal kidney tissue and the cdna fragment of hnadcs was produced by rt - pcr , then was purified and inserted into pgem - t vector . after dna sequenc - ing , pgem - hnadcs was digested with ecor i and sal i , and ? the dma fragment was subcloned into the ecor i and sal i sites of the fusion expression vector ( pgex - 5x - 1 )
从正常人肾组织中提取总rna ， rtpcr扩增hnadc3抗原表位区cdna片段，产物克隆至pgem吓载体，测序正确后，将hnadc3抗原表位区dna片段再次亚克隆至pgex融合表达载体，构建重组质粒pgex十nadc3 。 - The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others , especially by lixinlal in naigin normal university of china , we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence , using the favorable condon of the ecoli . after cheked the result of synthesis by sequence , we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ) , and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的内容包括：一、在前人对抗菌肽cmiv研究的基础上，对n端和c端进行氨基酸保守变换，设计和合成了该基因，充分使用大肠杆菌偏爱的密码子，并将该基因5端与硫氧还蛋白基因3端融合，通过ptxfus表达载体获得较高可溶性表达(在15 sds - page胶上可见明显的表达蛋白带) 。